Sanger Sequencing
Über den Vortrag
Der Vortrag „Sanger Sequencing “ von Georgina Cornwall, PhD ist Bestandteil des Kurses „Genomics“.
Quiz zum Vortrag
Which of the following components would be needed for Sanger dideoxy sequencing? Select all that apply.
- Dideoxynucleotides
- Regular DNA nucleotides
- Template strand
- DNA polymerase
- Taq polymerase
Sanger sequencing involves…?
- …the incorporation of a chain-terminating dideoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating dideoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-terminating deoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating deoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating dideoxyribonucleotide by DNA polymerase during in vitro DNA synthesis.
During enzymatic sequencing, DNA synthesis is terminated due to…?
- …the lack of a 3'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 5'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 2'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 1'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 4'-OH group on the ribose moiety of the dideoxynucleotide.
Which of the following steps are a part of automated sequencing? Select all that apply.
- Capillary gel electrophoresis
- Photodetection
- Use of fluorescent dye–labeled ddNTPs
- X-ray
- Autoradiography
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Dozent des Vortrages Sanger Sequencing
Georgina Cornwall, PhD
Dr. Georgina Cornwall is a Biology Instructor and currently works as an Instructional Designer in the private sector, developing interactive online training materials.
She obtained her PhD in Environmental, Population, and Organismic Biology at the University of Colorado, USA, in 2000, and has since taught a broad array of biology courses at various US universities.
She is an active promoter of hybrid/online teaching formats and interactive courses, and has received several awards for her engaging and innovative course design; e.g., she was named Adjunct Faculty of the Year at Colorado Mountain College in Aspen twice.
Within Lecturio, Dr. Cornwall teaches courses on Biology & Genetics.
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simply explained so well understood, very well done. lots of info but concise
