Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology
Über den Vortrag
Der Vortrag „Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology “ von Kevin Ahern, PhD ist Bestandteil des Kurses „Analytical Techniques in Biotechnology“.
Quiz zum Vortrag
Which of the following is true of 2D gel electrophoresis?
- Each spot on the final gel corresponds to a single protein.
- The first step involves separating proteins by molecular weight.
- SDS is necessary to separate proteins by pI.
- The smallest proteins are found at the top of the gel.
- The largest proteins are separated towards the negative charge.
In the 2D-gel analysis, the first step involves the separation of proteins according to which of the following options?
- The charge or pI value
- The mass of the protein
- The molecular weight of the protein
- The size of the protein
- The shape of the protein
What is the function of sodium dodecyl sulfate in the second step of 2d-gel analysis?
- It helps in the neutralization of all the charges present on each protein molecule under investigation.
- It helps in protecting the negatively charged amino acids on the proteins from the attack of cations present in the electrophoretic buffer.
- It helps in protecting the positively charged amino acids on the proteins from the attack of anions present in the electrophoretic buffer.
- It provides a charge to the neutral proteins so that these can pass easily through the gel column during electrophoresis.
- It protects the proteins from degradation and activity loss stimulated under the effect of electric current.
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Dozent des Vortrages Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology
Kevin Ahern, PhD
Dr. Kevin Ahern is a Professor in the Department of Biochemistry and Biophysics at Oregon State University (OSU), USA.
He obtained his PhD in Biochemistry from Oregon State University. Currently, he teaches courses for health sciences students at OSU.
He is co-author of three Open Educational electronic textbooks on Biochemistry and a Guide to Getting Into Medical School.
Due to his achievements, he earned OSU’s highest teaching recognition, the Elizabeth P. Ritchie Distinguished Professor Award in 2017.
Within Lecturio, Dr. Ahern teaches courses on Biochemistry and on "How to Get into Medical School”.
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VERY clear explanation of how this works, I love Ahern's lectures.
