DNA Amplification – Analytical Techniques in Biotechnology
About the Lecture
The lecture DNA Amplification – Analytical Techniques in Biotechnology by Kevin Ahern, PhD is from the course Analytical Techniques in Biotechnology.
Included Quiz Questions
Which of the following is true of the polymerase chain reaction (PCR)?
- It has three steps to each cycle.
- It requires an RNA primer for each round of replication.
- It works best with a heat-sensitive DNA polymerase.
- It typically amplifies DNA about 1000 fold.
- It requires transfer RNA.
Which of the following is true of the steps of the polymerase chain reaction (PCR) process?
- Denaturation is the first step.
- Renaturation must be performed very quickly.
- Elongation must be performed at temperatures close to boiling.
- The number of DNA sequences doubles every 5 cycles.
- Cold solutions are necessary for the reaction.
Which of the following is NOT a component of a PCR reaction mixture?
- RNA primer
- DNA template with target sequence
- Four types of dNTPs
- Taq polymerase
- DNA primers
Which of the following is NOT associated with PCR?
- Denaturation of template DNA — done with the help of thermostable helicase enzyme in the PCR mixture
- DNA primer — determines the replication start point during PCR
- Taq polymerase — a thermostable polymerase enzyme resistant to the denaturation step during PCR
- Thermal cycler — a device used to amplify the target DNA sequence via PCR
- Annealing temperature — temperature which affects the efficiency and specificity of PCR system by facilitating the hybridization of primer to the DNA template
According to the lecture, which of the following best describes the term 'omics'?
- The detection of genes, mRNA, proteins, and metabolites in a specific biological sample
- The replication of target genes in a very short time
- The synthesis of target mRNA with high accuracy and efficiency
- The synthesis of target tRNA and rRNA with high accuracy and efficiency
- The synthesis of the target protein and its related metabolites with high accuracy and efficiency
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Author of lecture DNA Amplification – Analytical Techniques in Biotechnology
Kevin Ahern, PhD
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It is an xcellent explication! I understend it at all. Thank you
