Serine Proteases – Enzyme Catalysis
About the Lecture
The lecture Serine Proteases – Enzyme Catalysis by Kevin Ahern, PhD is from the course Enzymes and Enzyme Kinetics.
Included Quiz Questions
Why is enzyme catalysis so effective?
- It lowers the energy of activation of a reaction.
- It changes the Gibbs free energy change of a reaction.
- Enzymes have constant, unmodulated activity.
- It allows reactions to go forward.
Which of the following is true of serine proteases?
- They use serine for catalysis in their active sites.
- They cut target proteins at serine residues.
- They bind serine during catalysis.
- They are misnamed because they do not involve serine at all.
The catalytic triad of the serine protease is composed of which of the following?
- Serine, histidine, and aspartic acid
- Serine, glutamic acid, and histidine
- Serine, glycine, and aspartic acid
- Serine, lysine, and histidine
- Serine, alanine, and selenocysteine
Which of the following site allows for the binding of an unstable intermediate during the proteolytic reaction of serine proteases?
- Oxyanion hole
- Active site
- Allosteric site
- S1 pocket
- Hydroxide/alkoxide pocket
Which of the following determines the specificity of the enzyme, serine protease?
- S1 pocket
- Oxyanion hole
- Active site
- Allosteric site
- Alkoxide/hydroxide pocket
Which of the following acts as a nucleophile during the catalytic action of a serine protease enzyme?
- Alkoxide ion produced on serine.
- N+ of histidine ring
- H+ of serine
- O- of aspartic acid
Which of the following is true about the mechanism of action of serine proteases?
- Aspartic acid and histidine play important roles.
- An electrophilic attack is at the core of the reaction mechanism.
- The slow step is the first one.
- The alkoxide ion forms on aspartic acid.
Which of the following is true of the S1 pocket of a serine protease?
- It determines the binding/cutting specificity of the enzyme.
- It stabilizes an unstable tetrahedral intermediate.
- It is the location of the catalytic triad.
- It requires serine for binding.
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Author of lecture Serine Proteases – Enzyme Catalysis
Kevin Ahern, PhD
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Attaractively and well explained video of serine protease catalytic mechanism that I have ever seen...Thank you..????
It is really helpful. I appreciate your kind efforts. Thanks verh much.
He simplified the concepts and illustrated by a very nice way
