Sanger Sequencing
About the Lecture
The lecture Sanger Sequencing by Georgina Cornwall, PhD is from the course Genomics.
Included Quiz Questions
Which of the following components would be needed for Sanger dideoxy sequencing? Select all that apply.
- Dideoxynucleotides
- Regular DNA nucleotides
- Template strand
- DNA polymerase
- Taq polymerase
Sanger sequencing involves…?
- …the incorporation of a chain-terminating dideoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating dideoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-terminating deoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating deoxynucleotide by DNA polymerase during in vitro DNA synthesis.
- …the incorporation of a chain-initiating dideoxyribonucleotide by DNA polymerase during in vitro DNA synthesis.
During enzymatic sequencing, DNA synthesis is terminated due to…?
- …the lack of a 3'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 5'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 2'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 1'-OH group on the ribose moiety of the dideoxynucleotide.
- …the lack of a 4'-OH group on the ribose moiety of the dideoxynucleotide.
Which of the following steps are a part of automated sequencing? Select all that apply.
- Capillary gel electrophoresis
- Photodetection
- Use of fluorescent dye–labeled ddNTPs
- X-ray
- Autoradiography
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Author of lecture Sanger Sequencing
Georgina Cornwall, PhD
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simply explained so well understood, very well done. lots of info but concise
