Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology by Kevin Ahern, PhD

Name: Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology
Uploaded: 2017-06-19
Duration: 6 min 26 s
Description: The lecture Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology by Kevin Ahern, PhD is from the course Analytical Techniques in Biotechnology.

About the Lecture

The lecture Proteomics (2d Gel Analysis) – Analytical Techniques in Biotechnology by Kevin Ahern, PhD is from the course Analytical Techniques in Biotechnology.

Included Quiz Questions

Which of the following is true of 2D gel electrophoresis?

Each spot on the final gel corresponds to a single protein.
The first step involves separating proteins by molecular weight.
SDS is necessary to separate proteins by pI.
The smallest proteins are found at the top of the gel.
The largest proteins are separated towards the negative charge.

In the 2D-gel analysis, the first step involves the separation of proteins according to which of the following options?

The charge or pI value
The mass of the protein
The molecular weight of the protein
The size of the protein
The shape of the protein

What is the function of sodium dodecyl sulfate in the second step of 2d-gel analysis?

It helps in the neutralization of all the charges present on each protein molecule under investigation.
It helps in protecting the negatively charged amino acids on the proteins from the attack of cations present in the electrophoretic buffer.
It helps in protecting the positively charged amino acids on the proteins from the attack of anions present in the electrophoretic buffer.
It provides a charge to the neutral proteins so that these can pass easily through the gel column during electrophoresis.
It protects the proteins from degradation and activity loss stimulated under the effect of electric current.