Molecular Cloning
About the Lecture
The lecture Molecular Cloning by Georgina Cornwall, PhD is from the course Biotechnology.
Included Quiz Questions
Which of the following are integral to the cloning technique? Select all that apply.
- The plasmid should have an origin of replication so that copies of the plasmid can be made.
- The plasmid needs an antibiotic-resistance gene to allow growth on an antibiotic-containing medium.
- The plasmid needs a gene with an observable phenotype that can be interrupted by the DNA of interest.
- The plasmid needs an antibiotic-resistance gene that can be interrupted by the DNA of interest.
- The plasmid must have an insulin-resistance gene so that it can break down glucose on the plated medium.
Which of the following best describes molecular cloning?
- The method of replication of a DNA molecule to produce a population of cells with identical DNA molecules
- The method of replication of a polysaccharide molecule to produce a population of cells containing polysaccharide molecules
- The method of replication of a protein molecule to produce a population of cells containing protein molecules
- The method of replication of a fatty acid molecule to produce a population of cells containing fatty acid molecules
- The method of replication of a phospholipid molecule to produce a population of cells containing phospholipid molecules
An E. coli cell was transformed with a recombinant plasmid, which was obtained after insertion of the human insulin gene in the lac Z gene on a plasmid (lac Z and ampicillin-resistance genes were initially present on the plasmid). The bacteria were plated on a medium containing ampicillin and X-gal. Which phenotypic characteristics would help identify colonies with the insulin gene integrated into the lac Z gene? Note: Active lac Z gene produces blue colonies and inactive lac Z gene produces white colonies.
- White bacterial colonies
- Blue bacterial colonies
- Pale-blue bacterial colonies
- Metallic-blue bacterial colonies
- Bacterial colonies with any color except white or blue
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Author of lecture Molecular Cloning
Georgina Cornwall, PhD
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