Microarray Analysis – Analytical Techniques in Biotechnology
About the Lecture
The lecture Microarray Analysis – Analytical Techniques in Biotechnology by Kevin Ahern, PhD is from the course Analytical Techniques in Biotechnology.
Included Quiz Questions
Which of the following is true of transciptomics?
- It can be performed with microarray analysis
- It tells how much protein is in a cell
- It focuses mostly on rRNA
- It focuses mostly on tRNA
Which of the following statements about microarray analysis of RNA is not true?
- RNA fragments are attached to a grid
- DNA fragments are attached to a grid
- Annealing of cDNA to the DNA is the key step in the process
- mRNAs are the RNAs being analyzed
Microarray analysis enables us to do which of the following?
- Determine the gene expressions and differentiate between cell types simultaneously on the single plate
- Determine the gene expressions and differentiate between cell types at the same time on the two different plates having different target spots on them
- Understand the relationship between DNA and RNA inside a dying cell
- Estimates the relative amounts of protein to DNA in a cell growing under stressed conditions
- Estimate the relative amounts of protein to RNA in a cell under normal or standard conditions
During a microarray analysis, the mRNA extracted from normal cells was tagged with the color green and cancer cells with the color red. Which of the following observations is FALSE regarding microarray analysis results?
- Bright blue color — mRNA expressed in both normal and cancer cells
- Bright green color — mRNA expressed in normal cells
- Bright red color — mRNA expressed in cancer cells
- Black color — mRNA expression absent in both normal and cancer cells
- Bright yellow color — mRNA expressed in both normal and cancer cells
In a microarray assay, the intensity and shade of the color on the grid indicates which of the following?
- Quantity and relative expression of the expressed transcriptome in the samples, respectively
- Quantity and relative expression of rRNA in the samples, respectively
- Quantity and relative expression of tRNA in the samples, respectively
- Relative expression and quantity of expressed mRNA in the samples, respectively
- Relative quantities of the cell membrane and nuclear membranes in the samples
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Author of lecture Microarray Analysis – Analytical Techniques in Biotechnology
Kevin Ahern, PhD
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Dr. Ahem explained a complex concept in a very interesting and simple way. Straight to the point and informative. Thank you
