Cutting the DNA
About the Lecture
The lecture Cutting the DNA by Georgina Cornwall, PhD is from the course Biotechnology.
Included Quiz Questions
Which of the following is TRUE regarding the recombination process? Select all that apply.
- Sticky ends are the regions of recombinant DNA that are spliced together.
- Palindromic sequences in DNA are recognized by restriction endonucleases to create sticky ends.
- Type III restriction endonuclease is the most precise.
- Restriction enzymes are most commonly used to insert bacterial DNA into human DNA.
- Sticky ends have to be methylated to attach to each other.
Which of the following statements is INCORRECT regarding the EcoRI enzyme?
- The EcoRI enzyme helps eukaryotic cells to become immunized against viral attacks.
- EcoRI is a useful restriction endonuclease enzyme for gene-cloning procedures.
- The action of the EcoRI enzyme results in dsDNA fragments having sticky ends with 5′ end overhangs of AATT.
- EcoRI enzyme cuts the G/AATTC recognition sequence on the dsDNA that has a palindromic complementary sequence CTTAA/G.
- EcoRI was the first restriction endonuclease enzyme isolated from E. coli.
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Author of lecture Cutting the DNA
Georgina Cornwall, PhD
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in depth knowledge on DNA splicing, ecor 1 and so the base pairs want to stick together and then ligase comes along to stick together the backbones, the phosphate-sugar backbones. Here you can see how the two pieces come together and now we have a recombinant DNA molecule. Again, we are generally looking at a circular chromosome and a foreign piece of DNA being inserted into that circular chromosome and so we'll see two ends of sticky ends and insertion of that sequence.
